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cell cycle staining solution  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Structured Review

    Multi Sciences (Lianke) Biotech Co Ltd cell cycle staining solution
    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
    Cell Cycle Staining Solution, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 98/100, based on 1462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+cycle+staining+solution/Cell+cycle+staining+Kit/pmc12963903-77-56-59
    Average 98 stars, based on 1462 article reviews
    cell cycle staining solution - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration"

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.051

    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
    Figure Legend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Techniques Used: In Vitro, Fluorescence, Staining

    Related Articles

    Staining:

    Article Title: Histone H3K36me2 demethylase KDM2A promotes bladder cancer progression through epigenetically silencing RARRES3
    Article Snippet: .. The cells were stained with 1 mL cell cycle staining solution (MULTI SCIENCES, Cat. No.70-CCS012) for 30 min on the ice in the dark. ..

    Article Title: Discovery of Kinetin in inhibiting colorectal cancer progression via enhancing PSMB1-mediated RAB34 degradation.
    Article Snippet: Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide.. Understanding the underlying mechanism driving CRC progression and identifying potential therapeutic drug targets are of utmost urgency.. We previously utilized LCMS-based proteomic profiling to identify proteins associated with postoperative progression in stage II/III CRC.

    Article Title: The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer
    Article Snippet: .. Cell cycle assay was constructed with Cell Cycle Staining Solution (Multi Sciences, China) according to the manufacturer’s protocol. .. The flow cytometry results were measured by the Beckman device (BD Biosciences, NJ, United States) and analyzed with Flow jo 10.0 software.

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection-Driven cartilage regeneration.
    Article Snippet: .. After LPS stimulation (10 μg mL− 1, 12 h, ServiceBio, GC205009) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 ◦C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark. .. DNA content was acquired on a BD LSRFortessa X-20 flow cytometer (excitation 488 nm), and cell-cycle phases were quantified in FlowJo.

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: .. After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark. .. DNA content was acquired on a BD LSRFortessa X-20 flow cytometer (excitation 488 nm), and cell-cycle phases were quantified in FlowJo.

    Article Title: The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer.
    Article Snippet: .. Cell cycle assay was constructed with Cell Cycle Staining Solution (Multi Sciences, China) according to the manufacturer’s protocol. .. The flow cytometry results were measured by the Beckman device (BD Biosciences, NJ, United States) and analyzed with Flow jo 10.0 software.

    Article Title: Overexpressed GNAZ predicts poor outcome and promotes G0/G1 cell cycle progression in hepatocellular carcinoma.
    Article Snippet: Aims: We aimed to investigate the role of G protein subunit alpha Z(GNAZ) in the progression and prognosis of patients with hepatocellular carcinoma (HCC).. Methods: Oncomine, GEO, TCGA, GEPIA2, Kaplan-Meier Plotter, TIMER2, Metascape, CCLE, LinkedOmics, and UALCAN databases were used to analyze the differential expression of GNAZ in HCC and normal liver tissues, relationship between GNAZ expression and prognosis of patients with HCC, and expression of GNAZ in common human HCC cell lines.. Western blotting was performed to analyze GNAZ expression, while the Cell Counting Kit 8 assay was used to determine cell proliferation, and flow cytometry was used to evaluate the cell cycle and apoptosis.

    Construct:

    Article Title: Discovery of Kinetin in inhibiting colorectal cancer progression via enhancing PSMB1-mediated RAB34 degradation.
    Article Snippet: Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide.. Understanding the underlying mechanism driving CRC progression and identifying potential therapeutic drug targets are of utmost urgency.. We previously utilized LCMS-based proteomic profiling to identify proteins associated with postoperative progression in stage II/III CRC.

    Article Title: The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer
    Article Snippet: .. Cell cycle assay was constructed with Cell Cycle Staining Solution (Multi Sciences, China) according to the manufacturer’s protocol. .. The flow cytometry results were measured by the Beckman device (BD Biosciences, NJ, United States) and analyzed with Flow jo 10.0 software.

    Article Title: The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer.
    Article Snippet: .. Cell cycle assay was constructed with Cell Cycle Staining Solution (Multi Sciences, China) according to the manufacturer’s protocol. .. The flow cytometry results were measured by the Beckman device (BD Biosciences, NJ, United States) and analyzed with Flow jo 10.0 software.

    Cell Cycle Assay:

    Article Title: The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer
    Article Snippet: .. Cell cycle assay was constructed with Cell Cycle Staining Solution (Multi Sciences, China) according to the manufacturer’s protocol. .. The flow cytometry results were measured by the Beckman device (BD Biosciences, NJ, United States) and analyzed with Flow jo 10.0 software.

    Article Title: The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer.
    Article Snippet: .. Cell cycle assay was constructed with Cell Cycle Staining Solution (Multi Sciences, China) according to the manufacturer’s protocol. .. The flow cytometry results were measured by the Beckman device (BD Biosciences, NJ, United States) and analyzed with Flow jo 10.0 software.

    Transfection:

    Article Title: Overexpressed GNAZ predicts poor outcome and promotes G0/G1 cell cycle progression in hepatocellular carcinoma.
    Article Snippet: Aims: We aimed to investigate the role of G protein subunit alpha Z(GNAZ) in the progression and prognosis of patients with hepatocellular carcinoma (HCC).. Methods: Oncomine, GEO, TCGA, GEPIA2, Kaplan-Meier Plotter, TIMER2, Metascape, CCLE, LinkedOmics, and UALCAN databases were used to analyze the differential expression of GNAZ in HCC and normal liver tissues, relationship between GNAZ expression and prognosis of patients with HCC, and expression of GNAZ in common human HCC cell lines.. Western blotting was performed to analyze GNAZ expression, while the Cell Counting Kit 8 assay was used to determine cell proliferation, and flow cytometry was used to evaluate the cell cycle and apoptosis.

    Control:

    Article Title: Overexpressed GNAZ predicts poor outcome and promotes G0/G1 cell cycle progression in hepatocellular carcinoma.
    Article Snippet: Aims: We aimed to investigate the role of G protein subunit alpha Z(GNAZ) in the progression and prognosis of patients with hepatocellular carcinoma (HCC).. Methods: Oncomine, GEO, TCGA, GEPIA2, Kaplan-Meier Plotter, TIMER2, Metascape, CCLE, LinkedOmics, and UALCAN databases were used to analyze the differential expression of GNAZ in HCC and normal liver tissues, relationship between GNAZ expression and prognosis of patients with HCC, and expression of GNAZ in common human HCC cell lines.. Western blotting was performed to analyze GNAZ expression, while the Cell Counting Kit 8 assay was used to determine cell proliferation, and flow cytometry was used to evaluate the cell cycle and apoptosis.



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    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
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    Multi Sciences (Lianke) Biotech Co Ltd permeabilization solution
    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
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    Image Search Results


    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark.

    Techniques: In Vitro, Fluorescence, Staining